本帖最后由 xxganxibao 于 2015-12-17 15:12 编辑 2 b5 l, U) f" ?" E, s$ W , M3 W2 w: {) a* b9 ]" A原文:P53L(L是上标,打不出来) and hGFAP-Cre mice have been described previously. PtenL(L也是上标) mice were generated using a standard knock-in approach in which Pten exon 5 is flanked by loxP sites. Mice were interbred and maintained on FvB/C57Bl6 hybrid background. ) y8 H3 _5 C/ V' p+ p + y5 q# W7 K& ~' Q- i9 n' ~" G这篇文章(P53 and Pten control neural and glioma stem/progenitor cell renewal and differentiation)讲的是P53和Pten都使用cre-lopx技术敲除的老鼠,有以下几个问题:0 ?$ c! Y. t v+ l, S, w; V
1. 上标的L是什么意思?一般看到的都是-/-这种代表等位基因上都敲除,也见到过L/L这种,是代表着Loxp的意思吗?单个的L是代表什么意思呢? - T4 U. X" O/ y- w, l2 g8 r2.Mice were interbred and maintained on FvB/C57Bl6 hybrid background这句话该如何理解?! o `- p/ c2 R# V( p3 W# j
3.这类敲除两个基因的老鼠是通过分别敲除一个基因的老鼠杂交得来的吗?能够稳定遗传这种性状吗? ! L4 l. }: O* M2 u7 @( d求大侠解答,不胜感激!作者: moluxingke 时间: 2015-12-17 17:07