PDF电子书：NanoCellBiology of Secretion

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NanoCellBiology of Secretion
  a5 J, h# U; w- W1 fImaging Its Cellular and Molecular Underpinnings
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NanoCellBiology of Secretion ....................................................................... 1' O, W3 G' S6 o: g/ _! e: R
Introduction ...................................................................................................... 19 |9 h- O# s) M
Materials and Methods ..................................................................................... 5
+ o" A& T* S- U$ w/ T6 u% UPancreatic Acini Isolation ............................................................................ 5
1 ^: M/ m6 K* `7 t1 j, \Zymogen Granule Isolation ......................................................................... 5( ~- i( K& _' b8 w, {
Isolation of Synaptosomes, Synaptosomal Membrane,
( ^. _7 G. J& T7 l/ s# _* g1 aand Synaptic Vesicles ................................................................................... 6  P3 G  \- x9 r( h4 R- M
Porosome Isolation ....................................................................................... 6
- L% e+ Z& E  e/ Y* T# l# [Atomic Force Microscopy ........................................................................... 7" N* r& n( e/ u
Measurement of Secretion ........................................................................... 7
- p" ~, E; T- i8 C5 r5 M% ZTransmission Electron Microscopy.............................................................. 8
( M: q0 A) ]# ^7 lDynamic Light Scattering (DLS) to Determine Vesicle
+ ~3 z# X3 Z9 h/ m8 A6 tVolume Change ............................................................................................ 8
* |/ z" J6 N& S7 p/ g7 n5 \Photon Correlation Spectroscopy ................................................................ 8
& {1 \4 J7 A" _. Q* ]+ oPorosome Functional Reconstitution Assay ................................................ 9
' [. O7 n, P# U3 n, _3 [Preparation of Liposomes and SNARE Reconstitutions .............................. 97 h( b( o; [3 j
Circular Dichroism Spectroscopy ................................................................ 10
) \$ z1 ]# i! x2 a" m( G( m, K, j. D2 dWide-Angle X-ray Diffraction ..................................................................... 10
+ u3 a6 V1 }5 K* V' Z0 K* gPorosome Discovery ........................................................................................ 11. T  y5 |1 g6 }2 @% h9 C6 n6 B" L% |
Calcium and SNARE-Induced Membrane Fusion ........................................... 23
  _% A$ Z/ l& F5 V! s  I& `- wv- and t-SNAREs Need to Reside in Opposing Membrane
0 z: J! P8 W  _# o0 Z# x+ ifor Appropriate Interactions and the Establishment
, p, R/ F1 B. \of Continuity Between Opposing Membranes ............................................. 26/ m5 E; ?3 ]7 C- D4 @+ R, B
Membrane Curvature Dictates the Size of the SNARE* i/ A4 J* g- I/ t
Ring Complex .............................................................................................. 31
5 R, F2 |* z/ e; CDisassembly of the t-/v-SNARE Complex .................................................. 326 g* `6 x8 e2 H' T# }! e; X
CD Spectroscopy Confi rms Membrane Requirement- T% G- e9 I' _$ @# u6 B1 {/ h7 J
for Appropriate t-/v-SNARE Assembly and Suffi ciency2 o' |( p# o+ C6 @  m0 E& H$ T; e7 Y
of NSF–ATP for Its Disassembly ................................................................. 38
0 U, d" x/ ?! ~4 o/ e8 gSNAREs Bring Opposing Bilayers into Close Approximation,, q2 K! n5 \, I" g
Enabling Calcium Bridging and Membrane Fusion .................................... 40
" p, k8 |0 Q) TMembrane Lipids Infl uence SNARE Complex0 q& Z2 J8 c/ E. w/ U0 X0 o% p
and Assembly–Disassembly ........................................................................ 42% v' m# ~6 i( ~3 h0 F
Secretion Involves Vesicle Swelling and Content Expulsion........................... 47
& e; {2 v: I2 l" V% Y) \" I2 wSecretory Vesicle Swelling Is Required for the Expulsion, O' M  U/ l8 E8 _' h1 K, d
of Intravesicular Contents During Cell Secretion ........................................ 473 F- l+ |% T' B- x+ J- E
Molecular Mechanism of Secretory Vesicle Swelling ................................. 53! }9 l1 t3 @" ]3 p( e
Presence of Functional b-Adrenergic Receptors at the Synaptic. I& F& M. [2 g0 C4 d
Vesicle Membrane ........................................................................................ 582 y6 @" E3 V) w2 r3 w
Conclusion ....................................................................................................... 623 {, h( }! n  M0 L& ?
References ........................................................................................................ 630 p6 y5 l* c; ~7 s+ Z; U, ^
Index ................................................................................................................ 693 t0 Y; X% s5 t1 F
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kouke80

好书。谢谢。

laoli1999

支持一下吧  

yukun

呵呵 高高实在是高~~~~~  

aakkaa

回帖是种美德.  

marysyq

不错 不错  比我强多了  

dypnr

鉴定完毕.!  

beautylive

我该不会是最后一个顶的吧  

陈晴

站个位在说  

依旧随遇而安

(*^__^*) 嘻嘻……