PDF电子书：NanoCellBiology of Secretion

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NanoCellBiology of Secretion4 U: g& o, ?9 H( ^3 f" q
Imaging Its Cellular and Molecular Underpinnings
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NanoCellBiology of Secretion ....................................................................... 1
) `! B. a+ x& S2 e- e' E  h4 m( KIntroduction ...................................................................................................... 1$ I8 k* O" w2 t5 ]6 K* l3 f
Materials and Methods ..................................................................................... 5. c# }, ^; `  d; O; ^$ L/ j1 R
Pancreatic Acini Isolation ............................................................................ 5
7 Q4 P5 N6 T4 }; kZymogen Granule Isolation ......................................................................... 5, B+ j$ H( [8 F5 T- L! A% M
Isolation of Synaptosomes, Synaptosomal Membrane,+ l+ @# J9 w6 O/ l. T
and Synaptic Vesicles ................................................................................... 60 I3 m6 R5 P/ n, ^% ], ^
Porosome Isolation ....................................................................................... 6
* u% T0 R4 t( f. ~! s" vAtomic Force Microscopy ........................................................................... 7
! G9 Q5 Z, R" N7 `8 eMeasurement of Secretion ........................................................................... 7; k) H! P5 V- j6 ~0 |6 t2 S
Transmission Electron Microscopy.............................................................. 8. n  v4 T! q) ?  u% Z
Dynamic Light Scattering (DLS) to Determine Vesicle
+ Q) [8 d% h* BVolume Change ............................................................................................ 8% |& `) p( |+ u8 E3 P2 i+ I
Photon Correlation Spectroscopy ................................................................ 83 j4 {& v0 R8 y) r$ r
Porosome Functional Reconstitution Assay ................................................ 9
+ @# u7 R1 R6 a8 V- DPreparation of Liposomes and SNARE Reconstitutions .............................. 9! r, Z' T/ c! I! y  r9 L3 F1 ]
Circular Dichroism Spectroscopy ................................................................ 106 |. U8 H' P1 T7 h4 T0 |
Wide-Angle X-ray Diffraction ..................................................................... 10! Y1 `8 o. R' C* m$ }9 u
Porosome Discovery ........................................................................................ 11
8 M- v1 _7 n/ _, ?5 R  ZCalcium and SNARE-Induced Membrane Fusion ........................................... 235 U$ `1 h- O: C( |/ A
v- and t-SNAREs Need to Reside in Opposing Membrane) b6 R$ Q+ b; j( j5 H' W; `& a
for Appropriate Interactions and the Establishment
$ ?8 i. C$ \1 t/ Hof Continuity Between Opposing Membranes ............................................. 26
' f' O) I/ t7 x8 }( \Membrane Curvature Dictates the Size of the SNARE
# _6 c, N% m. h- V1 \! J+ GRing Complex .............................................................................................. 31" J2 P- Y! R* Y) f. l4 Q
Disassembly of the t-/v-SNARE Complex .................................................. 32$ A- R3 K3 D/ M3 v. v0 P. i
CD Spectroscopy Confi rms Membrane Requirement8 B! ^* W& d$ @. P
for Appropriate t-/v-SNARE Assembly and Suffi ciency7 T- @9 _8 c: Z  @* i3 e2 u
of NSF–ATP for Its Disassembly ................................................................. 38& V0 j3 f5 e0 ~- T. W) \( q8 u
SNAREs Bring Opposing Bilayers into Close Approximation,5 Y  O9 i3 S, ]9 B  H5 u
Enabling Calcium Bridging and Membrane Fusion .................................... 40/ W2 k- h' f1 f7 N
Membrane Lipids Infl uence SNARE Complex
, x1 F# b' `) @) n5 B! oand Assembly–Disassembly ........................................................................ 42: d* n, L0 k& h& a4 n) v! M. R
Secretion Involves Vesicle Swelling and Content Expulsion........................... 47
1 r! K* ?( `7 O. b: u! pSecretory Vesicle Swelling Is Required for the Expulsion' `6 P5 a4 s6 T. \3 Y3 E% [
of Intravesicular Contents During Cell Secretion ........................................ 47
2 z* [9 ^6 y! @4 Z4 RMolecular Mechanism of Secretory Vesicle Swelling ................................. 53
* y! K# E7 T: a7 h8 [# H$ JPresence of Functional b-Adrenergic Receptors at the Synaptic- V/ A( @6 Q, s2 H% P
Vesicle Membrane ........................................................................................ 58
9 P, [5 _$ ^1 sConclusion ....................................................................................................... 623 c  ]5 M$ o+ p3 N
References ........................................................................................................ 63
( e0 `+ S1 j* ]Index ................................................................................................................ 69! @3 c1 q$ ^' l+ {$ p* O. k
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kouke80

好书。谢谢。

laoli1999

支持一下吧  

yukun

呵呵 高高实在是高~~~~~  

aakkaa

回帖是种美德.  

marysyq

不错 不错  比我强多了  

dypnr

鉴定完毕.!  

beautylive

我该不会是最后一个顶的吧  

陈晴

站个位在说  

依旧随遇而安

(*^__^*) 嘻嘻……