PDF电子书：NanoCellBiology of Secretion

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NanoCellBiology of Secretion
0 _. m6 n8 Z0 u1 ]5 LImaging Its Cellular and Molecular Underpinnings* H- Q  c+ t2 |% m3 @

8 m/ D3 N6 Q- J) V' Y7 g4 E# \- P6 S- BNanoCellBiology of Secretion ....................................................................... 1; D9 g. r7 E" S
Introduction ...................................................................................................... 10 Q0 z1 I  ^! J
Materials and Methods ..................................................................................... 5
2 b3 C5 J* p0 Z5 n$ X0 n# oPancreatic Acini Isolation ............................................................................ 5
: q+ g  Q# C7 NZymogen Granule Isolation ......................................................................... 5
$ C' ]7 _1 N- _. ]* ?6 t2 O9 Q# }$ ^Isolation of Synaptosomes, Synaptosomal Membrane,
+ B) w3 I2 k9 p# S, l5 U$ gand Synaptic Vesicles ................................................................................... 6
) V& b! O2 c7 N5 Z, WPorosome Isolation ....................................................................................... 6
3 |9 k& z* Y+ G$ @7 `. ~5 H! PAtomic Force Microscopy ........................................................................... 70 n3 w! M+ b( g0 Z
Measurement of Secretion ........................................................................... 7! ^+ @2 B6 Z4 w2 K& |* f# G8 J
Transmission Electron Microscopy.............................................................. 8: s  e  O2 W5 Y0 h# e* r
Dynamic Light Scattering (DLS) to Determine Vesicle
4 |. ^7 j. {- cVolume Change ............................................................................................ 8$ Q, I$ {8 \7 F4 `; A7 V: q6 \
Photon Correlation Spectroscopy ................................................................ 82 p  G1 o) S$ I
Porosome Functional Reconstitution Assay ................................................ 9
# b3 x7 Q% w0 e9 g0 P. S% P% |Preparation of Liposomes and SNARE Reconstitutions .............................. 9
/ m% j5 P3 c! Y  T# k% TCircular Dichroism Spectroscopy ................................................................ 10
' K; Y, _- U2 e. h3 [Wide-Angle X-ray Diffraction ..................................................................... 10
2 E$ [6 U* r  L1 n7 tPorosome Discovery ........................................................................................ 11* {, `8 t% E7 ^& Z5 b7 b
Calcium and SNARE-Induced Membrane Fusion ........................................... 23
8 q5 x, S" {* t! K( ov- and t-SNAREs Need to Reside in Opposing Membrane, K; ]! P; k. S( ^, s
for Appropriate Interactions and the Establishment
! g. C; w$ W3 T7 s. Y( O; v5 o# rof Continuity Between Opposing Membranes ............................................. 26
! o7 \7 w9 p/ B) {+ A1 [Membrane Curvature Dictates the Size of the SNARE) h& ]6 v( H  Q6 V" i9 t+ M  P
Ring Complex .............................................................................................. 31
* G3 X* b8 W6 W  j8 N: ~* C, UDisassembly of the t-/v-SNARE Complex .................................................. 32; J$ e1 p) w. F6 t
CD Spectroscopy Confi rms Membrane Requirement
- \: x% R6 v* R1 _* sfor Appropriate t-/v-SNARE Assembly and Suffi ciency
: s) V8 w& b# `0 B) |of NSF–ATP for Its Disassembly ................................................................. 38
$ b' Y" g4 P: F7 o* Q/ L. R+ J" k' RSNAREs Bring Opposing Bilayers into Close Approximation,& |& Q3 C) p2 C. o8 X
Enabling Calcium Bridging and Membrane Fusion .................................... 40" _1 H' J5 r( b5 O3 h
Membrane Lipids Infl uence SNARE Complex
, K1 m5 ]8 F* j5 f* _0 `6 Vand Assembly–Disassembly ........................................................................ 42
2 ~1 j9 \6 m- S1 Y( x3 gSecretion Involves Vesicle Swelling and Content Expulsion........................... 47
/ ], N: {0 D7 h! N( xSecretory Vesicle Swelling Is Required for the Expulsion3 V% m4 ?8 f% F$ X! o
of Intravesicular Contents During Cell Secretion ........................................ 47
( x& P8 a/ l8 _0 {' mMolecular Mechanism of Secretory Vesicle Swelling ................................. 53/ N. X/ A' E4 Q+ K) S, [$ g
Presence of Functional b-Adrenergic Receptors at the Synaptic8 `/ M9 N$ z0 E
Vesicle Membrane ........................................................................................ 58
( g% F5 f3 b4 j5 r# DConclusion ....................................................................................................... 62% Q" \. r: O! ]$ X
References ........................................................................................................ 63
) V! i  ~! l5 [2 M- P/ ^7 X3 rIndex ................................................................................................................ 69
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kouke80

好书。谢谢。

laoli1999

支持一下吧  

yukun

呵呵 高高实在是高~~~~~  

aakkaa

回帖是种美德.  

marysyq

不错 不错  比我强多了  

dypnr

鉴定完毕.!  

beautylive

我该不会是最后一个顶的吧  

陈晴

站个位在说  

依旧随遇而安

(*^__^*) 嘻嘻……