中国科学家首证iPS全能型的论文《iPS cells produce viable mice through tetraploid complementatio

芷瞻

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Nature advance online publication 23 July 2009 | doi:10.1038/nature08267; Received 9 January 2009; Accepted 3 July 2009; Published online 23 July 2009
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. l" \3 M" s. R. {iPS cells produce viable mice through tetraploid complementationnear-final version
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3 Z' h: p1 q: K& l8 @7 eXiao-yang Zhao1,2,5, Wei Li1,2,5, Zhuo Lv1,2,5, Lei Liu1, Man Tong1,2, Tang Hai1, Jie Hao1,2, Chang-long Guo1,2, Qing-wen Ma3, Liu Wang1, Fanyi Zeng3,4 & Qi Zhou11 w6 H  e! j' T8 X! P  V; `
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1.State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China 5 k; K2 E* G* D- S; _* y
2.Graduate School of Chinese Academy of Sciences, Beijing 100049, China
! _7 _/ M# |; e5 Y# I2 U  d3.Shanghai Institute of Medical Genetics, Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai 200040, China $ x' ]; D% \" u2 e& V3 |* U+ w" L
4.Institute of Medical Science, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
/ `, g" b. h  t7 k7 X) s# e0 K4 E5.These authors contributed equally to this work.
6 |0 M' z0 L( q) C7 x, t4 a/ NCorrespondence to: Fanyi Zeng3,4Qi Zhou1 Correspondence and requests for materials should be addressed to Q.Z. (Email: qzhou@ioz.ac.cn) or F.Z. (Email: fzeng@sjtu.edu.cn).
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Top of pageSince the initial description of induced pluripotent stem (iPS) cells created by forced expression of four transcription factors in mouse fibroblasts, the technique has been used to generate embryonic stem (ES)-cell-like pluripotent cells from a variety of cell types in other species, including primates and rat1, 2, 3, 4, 5, 6. It has become a popular means to reprogram somatic genomes into an embryonic-like pluripotent state, and a preferred alternative to somatic-cell nuclear transfer and somatic-cell fusion with ES cells7, 8. However, iPS cell reprogramming remains slow and inefficient. Notably, no live animals have been produced by the most stringent tetraploid complementation assay, indicative of a failure to create fully pluripotent cells. Here we report the generation of several iPS cell lines that are capable of generating viable, live-born progeny by tetraploid complementation. These iPS cells maintain a pluripotent potential that is very close to ES cells generated from in vivo or nuclear transfer embryos. We demonstrate the practicality of using iPS cells as useful tools for the characterization of cellular reprogramming and developmental potency, and confirm that iPS cells can attain true pluripotency that is similar to that of ES cells.6 P) e- p, F/ P
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http://www.nature.com/nature/jou ... pdf/nature08267.pdf
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芷瞻

听说，Zhou Qi 博士实验室的人来我们实验室学习过iPS技术；  \* g' g/ E+ B) c- S7 C( ]
人家回去后，发了牛文，可是，我们实验室都做了什么？？？- z1 k0 Z) A' q3 S  T

' ^, [# n$ l6 E5 a+ m* J, p呵呵，希望我们实验室也能早出牛文呀:)

刘实

能否把“多能”与“全能”的区别搞清楚再发言。

jianlindunev

请问老师你们是那个实验室的啊 ！我也想学IPS相关技术。不知道怎么联系

芷瞻

关于举办“干细胞iPS技术培训班”的第一轮通知
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7 g& U7 p! o% X0 Bhttp://124.16.10.3/ips/ips.html

water12006

哈哈---中国小牛人

随风的天使

感谢

arniekyo

无语了、

随风的天使

感谢

sniper

哪位大虾有这篇文章的翻译啊？跪求。。。。。。。。。。。。