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Pax3/7BP Is a Pax7- and Pax3-Binding Protein that Regulates the Proliferation of Muscle Precursor Cells by an Epigenetic Mechanism生物通 www.ebiotrade.com
6 ?; v# l# T4 |, q) p7 bIn mouse skeletal muscles, Pax7 uniquely marks muscle satellite cells and plays some important yet unknown functions at the perinatal stage. To elucidate its in vivo functions, we initiated a yeast two-hybrid screening to look for Pax7-interacting proteins and identified a previously uncharacterized Pax7- and Pax3-binding protein (Pax3/7BP). Pax3/7BP is a ubiquitously expressed nuclear protein, enriched in Pax7+ muscle precursor cells (MPCs), and serves as an indispensable adaptor for Pax7 to recruit the histone 3 lysine 4 (H3K4) methyltransferase (HMT) complex by bridging Pax7 and Wdr5. Knockdown of Pax3/7BP abolished the Pax3/7-associated H3K4 HMT activity and inhibited the proliferation of Pax7+ MPCs from young mice both in culture and in vivo. Id3 and Cdc20 were direct target genes of Pax7 and Pax3/7BP involved in the proliferation of Pax7+ MPCs. Collectively, our work establishes Pax3/7BP as an essential adaptor linking Pax3/7 with the H3K4 HMT to regulate the proliferation of MPCs.2 D6 m" E( a: g
作者简介:
# r) h. U4 a5 z/ H5 v, k2 c7 \' C邬振国
' [0 f2 P6 ~$ w) A香港科技大学生命科学院教授,深圳北京大学香港科技大学医学中心生物医学研究所合作导师。 7 h: {- Q1 m/ g5 {2 p# ^
1986年毕业于南京大学生物化学系,1995年于加拿大西安大略大学(University of Western Ontario)生物化学系获得生化博士学位,并于1996-1999在加州大学圣地亚哥分校师从Prof. Michael Karin做博士后,从事促分裂原活化蛋白激酶(MAPK)的信号传导研究。1999年起在香港科技大学任助理教授,目前为生物化学系教授。2004年获中国自然科学基金会海外杰出青年基金,目前主要研究工作为研究不同信号传导通路在骨骼肌分化过程中的作用以及肿瘤细胞中的信号传导通路。先后在Genes Dev., EMBO J., J. Biol. Chem., Mol. Cell Biol., J. Cell. Sci.等国际著名期刊上发表论文。 |
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