PDF 电子书：Animal Cell Biotechnology, 第2版，2007年新版

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附加上目录，以供需要的人参考。
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Contents: b# k- \/ b1 [- j. w! u3 M
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Preface ........................................................................................................v
! Y/ t# C& y# N7 A) NContributors ............................................................................................ xiii
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7 K2 J/ H! h* wPART I. BASIC TECHNIQUES FOR ESTABLISHING PRODUCTION CELL LINES
9 q3 x+ s5 o" V& y6 a$ t" U6 d1 Transduction Technologies
, \, c' g* g, o* _! `David A. Fluri and Martin Fussenegger ........................................... 3
! H# [0 S2 R" r) {; p+ ]" p2 Cells for Gene Therapy and Vector Production Christophe Delenda, Miguel Chillon, Anne-Marie Douar,7 E/ l' v. ^9 j  ]3 Z# @) c. K5 g
and Otto-Wilhelm Merten ........................................................ 23. P% o' L3 r3 p5 ^/ W
3 Technology and Production of Murine Monoclonal and Recombinant Antibodies and Antibody Fragments
& ?. A) C+ i2 T3 w- f0 }. sAlexandra Dorn-Beineke, Stefanie Nittka, and Michael Neumaier .............................................................. 93- n! }. v7 E2 O6 Z, Q$ B; _& P7 ]
4 DNA Fingerprinting and Characterization of Animal Cell Lines$ X+ @4 U$ [1 t  x; L9 {) {3 E7 ~
Glyn N. Stacey, Ed Byrne, and J. Ross Hawkins ......................... 123
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1 L! H. u8 f6 P* [* f: x5 b( K- WPART II. BASIC CULTIVATION TECHNIQUES
( B) h: v7 L- N9 z. x( {% }5 Microcarrier Cell Culture Technology$ I  G& U% o" Q$ o5 k" ^4 y
Gerald Blüml ............................................................................... 149
2 p/ I$ M" O7 y- c5 w$ B/ c6 Cell Encapsulation
" \' {8 o3 j  UHolger Hübner ............................................................................ 1798 }% R$ T! ^) E8 W* r* J
7 Tools for High-Throughput Medium and Process Optimization
  F: H2 f9 U5 E' iMartin Jordan and Nigel Jenkins ................................................. 1938 v+ ^! |; D+ w7 s% h. f( S
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PART III. CELL CHARACTERIZATION AND ANALYSIS
( r9 [/ x9 l# ^4 ^4 S/ {' w8 {8 Cell Counting and Viability Measurements Michael Butler and Maureen Spearman...................................... 205# @8 U% [  b" a' x% D0 u' E
9 Monitoring of Growth, Physiology, and Productivity of Animal Cells by Flow Cytometry
. o) b1 V) c9 E' t  F7 ZSilvia Carroll, Mariam Naeiri, and Mohamed Al-Rubeai ............ 223# i" f* g. z1 C( ]) W
10 Nuclear Magnetic Resonance Methods for Monitoring Cell Growth and Metabolism in Intensive Bioreactors
8 J! ~) ]+ ~& r) c% k$ XAndré A. Neves and Kevin M. Brindle ........................................ 239
, @  m/ k" _# U11 Methods for Off-Line Analysis of Nutrients and Products in Mammalian Cell Culture
7 c2 s6 W. b% l$ aHeino Büntemeyer....................................................................... 253
) T: j) ~' k8 P: ~. r; m12 Application of Stoichiometric and Kinetic Analyses to Characterize Cell Growth and Product Formation  ^* L  s  F$ b, s- V
Derek Adams, Rashmi Korke, and Wei-Shou Hu ........................ 269- p$ @# r. H: k! `* i
13 Measurement of Apoptosis in Cell Culture" @9 l% s& {9 |2 ?( ?
Adiba Ishaque and Mohamed Al-Rubeai ..................................... 2852 Z1 V  q/ g9 ~0 T1 V, L
14 Metabolic Flux Estimation in Mammalian Cell Cultures
1 E* J5 v+ ]  e* r( v5 aChetan T. Goudar, Richard Biener, James M. Piret, and Konstantin B. Konstantinov .............................................. 3018 X. {+ U; x/ T! W+ C. ?
PART IV. SPECIAL CULTIVATION TECHNIQUES
3 J) b% Y$ S$ q0 g7 D3 \15 Disposable Bioreactors for Inoculum Production and Protein Expression
/ Y& O% i; j9 VRegine Eibl and Dieter Eibl .......................................................... 321
/ D3 V* I  s3 {$ X) ~9 y' t16 Hollow Fiber Cell Culture! R1 R7 }  F. N! h4 _8 @0 x
John M. Davis .............................................................................. 337
7 q6 U7 C% j+ j) S) g" [17 Cultivation of Mammalian Cells in Fixed-Bed Reactors
5 {6 }0 n/ }/ F  nRalf Pörtner and Oscar B. J. Platas Barradas .............................. 353
6 D: Q6 j; h; H( u: @9 N18 Configuration of Bioreactors8 K9 M5 A2 _6 @
Dirk E. Martens and Evert Jan van den End ................................ 371* I' C8 L- d5 A- V

& K1 u5 B% k; n  yPART V. DOWNSTREAM TECHNIQUES- a$ c& f+ S8 W& y
19 Membrane Filtration in Animal Cell Culture% a6 j) T) p1 p
Peter Czermak, Dirk Nehring, and Ranil Wickramasinghe ........ 397
8 f' w: W: ~, ]9 |20 Chromatographic Techniques in the Downstream Processing
+ e8 z1 o8 l3 nof (Recombinant) Proteins
, k, P0 \1 @# `6 g6 w7 QRuth Freitag ................................................................................. 421
; \. x/ D! ~" jPART VI. SPECIAL APPLICATIONS  O4 z1 L( |: I- J  X1 N# |
21 Vaccine Production: State of The Art and Future Needs
/ @5 O2 P; s1 r: ein Upstream Processing3 U) }  o' V, ]: R
Yvonne Genzel and Udo Reichl .................................................. 457& Z2 f8 Q5 u) p4 N# e! d' G
22 Retrovirus Production and Characterization
. ]/ P. O. R: Q4 ePedro E. Cruz, Marlene Carmo, Teresa Rodrigues,0 |) M+ _- l2 q' Y
and Paula Alves ....................................................................... 475% W/ Z' Z4 D# w. H
23 Insect Cell Cultivation and Generation of Recombinant6 X& B3 U6 Z1 j# {; v
Baculovirus Particles for Recombinant Protein Production' c' F! K6 z2 y' _2 M
Sabine Geisse ............................................................................... 489
' Y# A1 e% b. {3 W! T. G& M( {9 XIndex ...................................................................................................... 509
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