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本帖最后由 细胞海洋 于 2011-3-31 14:38 编辑 0 V1 H8 S' \% ?. t. A1 q
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附加上目录，以供需要的人参考。% K8 V9 s3 M3 @' ?0 ]
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Contents# ?- D9 s; ]; G& W0 x7 D5 E
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Preface ........................................................................................................v' v2 v( r( L8 u9 Y
Contributors ............................................................................................ xiii
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4 a2 k- Q* n% u; EPART I. BASIC TECHNIQUES FOR ESTABLISHING PRODUCTION CELL LINES
! O$ T+ |4 H/ ]; n1 Transduction Technologies7 l1 s" j- M" j- S# O( Q5 _9 ~* C
David A. Fluri and Martin Fussenegger ........................................... 3( o; w$ Q0 \0 E0 w6 o
2 Cells for Gene Therapy and Vector Production Christophe Delenda, Miguel Chillon, Anne-Marie Douar,
L9 X2 Y' T( o- O: fand Otto-Wilhelm Merten ........................................................ 23
! Y$ b3 T& C. r- y3 Technology and Production of Murine Monoclonal and Recombinant Antibodies and Antibody Fragments2 o* }- x6 e9 p5 ` F2 w3 U
Alexandra Dorn-Beineke, Stefanie Nittka, and Michael Neumaier .............................................................. 93
3 N* G: t2 u, W% J a) S4 DNA Fingerprinting and Characterization of Animal Cell Lines
+ F) |+ h; I& MGlyn N. Stacey, Ed Byrne, and J. Ross Hawkins ......................... 123
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b$ \5 L% h& X5 A* GPART II. BASIC CULTIVATION TECHNIQUES
* Y5 X& l$ `8 W. F+ i9 `" u' f5 Microcarrier Cell Culture Technology
3 q% h5 f3 k( M' U) c/ ?Gerald Blüml ............................................................................... 1490 y( f* Y; y6 c. P$ u- k
6 Cell Encapsulation
+ W* X( X+ x* j0 k5 g: S8 R% @- eHolger Hübner ............................................................................ 179
; r% S: O: O! @3 x. W7 Tools for High-Throughput Medium and Process Optimization' U( Z! r1 I o3 B; ?
Martin Jordan and Nigel Jenkins ................................................. 193- A; Y* i5 v5 u7 \7 m* C# ?
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PART III. CELL CHARACTERIZATION AND ANALYSIS
0 p# c) u( B+ m. m8 Cell Counting and Viability Measurements Michael Butler and Maureen Spearman...................................... 205+ j% w3 e3 l1 B" T- g
9 Monitoring of Growth, Physiology, and Productivity of Animal Cells by Flow Cytometry% g/ R9 N9 O$ L5 O! I0 D
Silvia Carroll, Mariam Naeiri, and Mohamed Al-Rubeai ............ 223, D, t" Q: Y+ G
10 Nuclear Magnetic Resonance Methods for Monitoring Cell Growth and Metabolism in Intensive Bioreactors( `! H5 r: I" \. i T
André A. Neves and Kevin M. Brindle ........................................ 239
4 H+ o" Y8 }" f4 Z. m9 a( p5 b11 Methods for Off-Line Analysis of Nutrients and Products in Mammalian Cell Culture$ r4 g5 j0 e b6 b( f
Heino Büntemeyer....................................................................... 253
# M9 D" Y0 U3 i) I7 X( H12 Application of Stoichiometric and Kinetic Analyses to Characterize Cell Growth and Product Formation6 |. [* v& R) |6 |! |4 z" l
Derek Adams, Rashmi Korke, and Wei-Shou Hu ........................ 269. \! a6 h6 h; ~
13 Measurement of Apoptosis in Cell Culture
2 E( H0 F. B" V: V2 N5 U# zAdiba Ishaque and Mohamed Al-Rubeai ..................................... 285
3 g+ J7 e, ~8 |- E' k& ? t14 Metabolic Flux Estimation in Mammalian Cell Cultures
% E3 f+ U6 x ^! OChetan T. Goudar, Richard Biener, James M. Piret, and Konstantin B. Konstantinov .............................................. 301
Q1 \% l6 Z$ f. ?7 q6 oPART IV. SPECIAL CULTIVATION TECHNIQUES
) D$ t4 [1 c. T3 J6 j15 Disposable Bioreactors for Inoculum Production and Protein Expression
! k" f& {# @; M, ?/ v# s- }9 HRegine Eibl and Dieter Eibl .......................................................... 321/ l7 p4 }+ g3 b: X3 ~
16 Hollow Fiber Cell Culture4 X' K4 w" w& `7 D
John M. Davis .............................................................................. 3379 D5 Y9 s* }6 J% e- p* `
17 Cultivation of Mammalian Cells in Fixed-Bed Reactors
, {9 l* E' j$ {5 @$ _. b$ U" TRalf Pörtner and Oscar B. J. Platas Barradas .............................. 353
0 ^/ i% {$ ~7 Y3 p18 Configuration of Bioreactors9 w) v) C+ ?* |) w
Dirk E. Martens and Evert Jan van den End ................................ 371
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PART V. DOWNSTREAM TECHNIQUES1 g4 ]$ `4 k* T: y( b
19 Membrane Filtration in Animal Cell Culture
+ {( ]# l: X* y0 H" ^Peter Czermak, Dirk Nehring, and Ranil Wickramasinghe ........ 397
f+ Z( ?6 u; |1 w L. l/ u7 P20 Chromatographic Techniques in the Downstream Processing
. c2 ~$ g2 `5 C, Rof (Recombinant) Proteins
2 R, l* }' c5 F, xRuth Freitag ................................................................................. 421
3 J6 s$ o6 Q; H7 \PART VI. SPECIAL APPLICATIONS
: s( {4 T" D+ y6 A* I21 Vaccine Production: State of The Art and Future Needs, A' L: W1 b* U
in Upstream Processing
$ h7 m5 h5 E: {$ Y2 s) C2 xYvonne Genzel and Udo Reichl .................................................. 457. X0 n/ ?0 {6 W. l; d" o
22 Retrovirus Production and Characterization8 T! _% l x# q; m( c! I% _
Pedro E. Cruz, Marlene Carmo, Teresa Rodrigues,
8 h* X8 s/ B5 r) Z+ I4 _- ?and Paula Alves ....................................................................... 475
" q) {, d! T2 C8 v6 ?5 ]) D23 Insect Cell Cultivation and Generation of Recombinant
; U" Y3 T$ L5 o0 f. PBaculovirus Particles for Recombinant Protein Production7 R% G! n8 x' d- H1 f0 F& i
Sabine Geisse ............................................................................... 489 Q) l) r3 Y0 m6 s F7 y
Index ...................................................................................................... 509; g' S3 A9 @5 b& l
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发表于 2011-3-31 12:51
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