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Current Protocols in Stem Cell Biology 2007年完整版 1451页
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Online ISBN:9780470151808( c- ~2 @3 @! C% f. j7 t2 B
DOI: 10.1002/9780470151808
Table of Contents, k$ [+ y4 O) ?
1. Foreword
2. Preface
3. Chapter 1 Embryonic and Extraembryonic Stem Cells
1. Section A Isolation of Embryonic Stem Cells9 u! `8 U5 n& {3 r( U
1. Introduction" L0 {+ q; v& Y
2. Unit 1A.1 Derivation and Characterization of Nonhuman Primate Embryonic Stem Cells! |2 c( N2 u9 ^$ v9 J( }
3. Unit 1A.2 Derivation of hESC from Intact Blastocysts
4. Unit 1A.3 Reprogramming Primordial Germ Cells (PGC) to Embryonic Germ (EG) Cells9 }1 i# X( n6 O: B% ]( v0 u5 c& D0 p: Z
5. Unit 1A.4 Derivation and Propagation of hESC Under a Therapeutic Environment
2. Section B Characterization of Embryonic Stem Cells+ C# C& W9 F3 ~8 Z$ F: y6 J3 }
1. Unit 1B.1 Proteomic Analysis of Pluripotent Stem Cells9 t3 n0 n- I; i% v0 u
2. Unit 1B.2 Gene Expression Analysis of RNA Purified from Embryonic Stem Cells and
Embryoid Body–Derived Cells Using a High-Throughput Microarray Platform
3. Unit 1B.3 Phenotypic Analysis of Human Embryonic Stem Cells$ a7 ~. M: H$ B6 `2 k! q8 o, Q
4. Unit 1B.4 Isolation of Human Embryonic Stem Cell–Derived Teratomas for the Assessment of
Pluripotency
5. Unit 1B.5 Tandem Affinity Purification of Protein Complexes in Mouse Embryonic Stem Cells
Using In Vivo Biotinylation" J4 K9 J9 b7 F' G, m
6. Unit 1B.6 Characterization of X-Chromosome Inactivation Status in Human Pluripotent Stem2 s- V+ w4 T2 z6 U
Cells
7. Unit 1B.7 Preparation of Defined Human Embryonic Stem Cell Populations for Transcriptional9 L6 E( M% X9 z  G) e
Profiling+ w$ y+ C; }$ @9 E, m5 I: t6 U3 G
3. Section C Culture and Maintenance of Undifferentiated Embryonic Stem Cells
1. Introduction) o$ l0 A/ O3 P* \8 C$ Q0 W
2. Unit 1C.1 Expansion of Human Embryonic Stem Cells In Vitro
3. Unit 1C.2 Defined, Feeder-Independent Medium for Human Embryonic Stem Cell Culture: A' {. a+ @4 o- q# E! P
4. Unit 1C.3 Isolation and Propagation of Mouse Embryonic Fibroblasts and Preparation of7 g; Y* n4 e1 X0 H3 k6 P
Mouse Embryonic Feeder Layer Cells/ m3 U; L' O7 _* `. H7 @( h# Z( C
5. Unit 1C.4 Culture of Mouse Embryonic Stem Cells
6. Unit 1C.5 Preparation of Autogenic Human Feeder Cells for Growth of Human Embryonic
Stem Cells
7. Unit 1C.6 Isolation of Human Placental Fibroblasts/ V& e0 a0 [; H# m2 E7 Q$ a
8. Unit 1C.7 Derivation of Human Skin Fibroblast Lines for Feeder Cells of Human Embryonic
Stem Cells
9. Unit 1C.8 Cryopreservation of Dissociated Human Embryonic Stem Cells in the Presence of: X0 N1 a% `. d+ x4 v# v" {
ROCK Inhibitor9 W0 k4 C( R7 a) {" c
10. Unit 1C.9 Authentication and Banking of Human Pluripotent Stem Cells
11. Unit 1C.10 Clump Passaging and Expansion of Human Embryonic and Induced Pluripotent
Stem Cells on Mouse Embryonic Fibroblast Feeder Cells
12. Unit 1C.11 Expansion of Human Embryonic Stem Cells on Cellulose Microcarriers/ W# S) u+ a+ c. m' E
4. Section D Germ Layer Induction/Differentiation of Embryonic Stem Cells8 V3 R, Q1 H0 v. f8 D/ l+ g- \! r
1. Unit 1D.1 Germ Layer Induction in ESC—Following the Vertebrate Roadmap; p& x% E2 G6 ]6 H1 q! z
2. Unit 1D.2 Formation and Hematopoietic Differentiation of Human Embryoid Bodies by
Suspension and Hanging Drop Cultures
3. Unit 1D.3 Directed Differentiation of Human Embryonic Stem Cells as Spin Embryoid Bodies" u1 H0 p( X; K& J2 Y
and a Description of the Hematopoietic Blast Colony Forming Assay
4. Unit 1D.4 Differentiation of Human Embryonic Stem Cells in Adherent and in Chemically5 ]% G4 P# a! r8 t1 F5 K1 s
Defined Culture Conditions5 s& _" T9 l$ W$ @
5. Unit 1D.5 Isolation and Differentiation of Xenopus Animal Cap Cells
5. Section E Extraembryonic Lineages# X2 V$ R4 q1 d9 Q5 F0 e: V
1. Introduction
2. Unit 1E.1 Isolation of Human Placenta-Derived Multipotent Cells and In Vitro Differentiation2 R) E& J3 ~, A. C$ A2 _8 }8 S  r
into Hepatocyte-Like Cells- w  _* m% ?, p
3. Unit 1E.2 Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta1 }4 i/ y. E/ y: e5 _6 q. m$ l
4. Unit 1E.3 Isolation of Amniotic Epithelial Stem Cells
5. Unit 1E.4 Isolation and Manipulation of Mouse Trophoblast Stem Cells
6. Unit 1E.5 Isolation of Amniotic Mesenchymal Stem Cells$ r$ H* E( C. {% Q
7. Unit 1E.6 Amnion Epithelial Cell Isolation and Characterization for Clinical Use
6. Section F Mesodermal Lineages; Q+ D0 w; Z  `+ z. @4 V
1. Unit 1F.1 Differentiation of Embryonic Stem Cells into Cartilage Cells/ T. i' [2 C6 C8 }& M2 s) {
2. Unit 1F.2 Differentiation of Human Embryonic Stem Cells to Cardiomyocytes by Coculture
with Endoderm in Serum-Free Medium$ r" Z, h# ?! f
3. Unit 1F.3 Isolation of Hematopoietic Stem Cells from Mouse Embryonic Stem Cells' t! }6 H6 e/ ?  P
4. Unit 1F.4 Differentiation of Mouse Embryonic Stem Cells into Blood. k4 v  Q! R3 C' S/ c. T, b* t
5. Unit 1F.5 Endothelial Differentiation of Embryonic Stem Cells
6. Unit 1F.6 Hematopoietic Differentiation of Human Embryonic Stem Cells by Cocultivation0 x/ m, p9 k+ ^6 k! V- z
with Stromal Layers
7. Unit 1F.7 TLX1 (HOX11) Immortalization of Embryonic Stem Cell–Derived and Primary" Q& |- F5 G& n' ]7 C
Murine Hematopoietic Progenitors' R5 u* s2 i1 {% U* ^) N/ L
8. Unit 1F.8 Differentiation of Multipotent Mesenchymal Precursors and Skeletal Myoblasts from& ]2 B9 X* f' r- P
Human Embryonic Stem Cells1 [& {7 @% ~! c& f  {6 g
9. Unit 1F.9 Derivation of Vasculature from Embryonic Stem Cells
10. Unit 1F.10 Isolation and Functional Characterization of Pluripotent Stem Cell–Derived Cardiac9 w# I8 S4 S0 _9 Q! M  c2 Q: b, @5 I
Progenitor Cells- o8 k. O1 ?/ J1 E# ^* ^# u" O
11. Unit 1F.11 Differentiation of Mouse Embryonic Stem Cells into Cardiomyocytes via the; C) j+ ^8 _" N* R
Hanging-Drop and Mass Culture Methods  `; N- ], z7 ~" |' @  s/ Z4 A
7. Section G Endodermal Lineages. W+ `7 @, U/ Q
1. Unit 1G.1 The Differentiation of Distal Lung Epithelium from Embryonic Stem Cells+ y& x3 B6 h2 @8 M- x8 u5 }
2. Unit 1G.2 Pancreas Differentiation of Mouse ES Cells
3. Unit 1G.3 Differentiation of Embryonic Stem Cells into Anterior Definitive Endoderm! V: `# Q$ z4 j% ^
8. Section H Ectodermal Lineages
1. Unit 1H.1 Differentiation of Mouse Embryonic Stem Cells to Spinal Motor Neurons2 u5 e6 N/ c, g+ F" W
2. Unit 1H.2 Time-Lapse Imaging of Embryonic Neural Stem Cell Division in Drosophila by5 W+ @5 I8 V  c
Two-Photon Microscopy
4. Chapter 2 Somatic Stem Cells
1. Section A Hematopoietic Stem Cells
1. Introduction' y+ l1 w' |8 ?. p
2. Unit 2A.1 Isolation of Mononuclear Cells from Human Cord Blood by Ficoll-Paque Density
Gradient" i* o+ S, {: [) _1 g; t
3. Unit 2A.2 Isolation of Hematopoietic Stem Cells from Human Cord Blood8 I' B$ b9 c8 x) h% |4 \
4. Unit 2A.3 Isolation of Mesenchymal Stem Cells from Human Cord Blood* s1 j& _) c7 d
5. Unit 2A.4 Isolation and Assessment of Long-Term Reconstituting Hematopoietic Stem Cells
from Adult Mouse Bone Marrow
6. Unit 2A.5 Analysis of the Hematopoietic Stem Cell Niche# R4 C  t3 h2 G# a9 g
7. Unit 2A.6 Analysis and Manipulation of Hematopoietic Progenitor and Stem Cells from Murine  g- K+ p6 E6 e
Embryonic Tissues: D  l# a& Q: y% t/ f: L- H
8. Unit 2A.7 High Level In Vitro Expansion of Murine Hematopoietic Stem Cells3 ]) k1 a4 u4 L6 T
9. Unit 2A.8 Isolation and Visualization of Mouse Placental Hematopoietic Stem Cells
10. Unit 2A.9 Preparation of Hematopoietic Stem and Progenitor Cells from the Human Placenta
2. Section B Non-Hematopoietic Bone Marrow-Derived Stem Cells
1. Unit 2B.1 Isolation and Characterization of Mesoangioblasts from Mouse, Dog, and Human! O  \$ [+ I' @- ]% c, ~2 f% @
Tissues; }1 ]* M' C! t: P6 y5 d
2. Unit 2B.2 Purification and Culture of Human Blood Vessel–Associated Progenitor Cells
3. Unit 2B.3 Isolation, Culture, and Differentiation Potential of Mouse Marrow Stromal Cells9 u' I2 l# |7 r, Z
3. Section C Cardiovascular Stem Cells* S9 l: g, ~* z9 C7 u
1. Unit 2C.1 Isolation and Characterization of Endothelial Progenitor Cells from Human Blood
2. Unit 2C.2 Derivation of Epicardium-Derived Progenitor Cells (EPDCs) from Adult Epicardium' @6 o& ]; j0 v  z5 e- `
3. Unit 2C.3 Isolation and Expansion of Cardiosphere-Derived Stem Cells' p" j  \. i# D: j
4. Section D Neural Stem Cells+ X7 {2 B, s. X. x, M5 h+ w0 e0 A
1. Unit 2D.1 Generation of Cultured Oligodendrocyte Progenitor Cells from Rat Neonatal Brains. \# p& S& X' G& S
2. Unit 2D.2 Isolating, Expanding, and Infecting Human and Rodent Fetal Neural Progenitor Cells
3. Unit 2D.3 Long-Term Multilayer Adherent Network (MAN) Expansion, Maintenance, and
Characterization, Chemical and Genetic Manipulation, and Transplantation of Human Fetal. `0 ]8 n4 c8 l  J
Forebrain Neural Stem Cells- e. B" X+ D$ ~3 i% V* I
4. Unit 2D.4 Culture System for Rodent and Human Oligodendrocyte Specification, Lineage1 L% A/ N8 `% E) s9 ~+ g! K! w: U( ~9 b
Progression, and Maturation. v, u- W1 b& m* U& f; O
5. Unit 2D.5 Isolation and Culture of Ventral Mesencephalic Precursor Cells and Dopaminergic
Neurons from Rodent Brains+ R; c* X8 [+ [) x
6. Unit 2D.6 Isolation of Neural Stem Cells from Neural Tissues Using the Neurosphere9 T7 i# X9 n8 q# t2 B0 s+ g5 L# s
Technique
5. Section E Germline Stem Cells
1. Unit 2E.1 Culturing Ovarian Somatic and Germline Stem Cells of Drosophila! W1 L+ z% }- Q' u& S$ y
2. Unit 2E.2 Time-Lapse Live Imaging of Stem Cells in Drosophila Testis' C+ B; x1 q3 a' {, s* z6 h2 E
6. Section F Gut Stem Cells; M/ |& v+ c- \& z& B
1. Unit 2F.1 In Situ Hybridization to Identify Gut Stem Cells3 z; f/ e, `6 r& Y. o# v; J
7. Section G Lung Stem Cells' E8 J0 x% C6 g  w# b
1. Unit 2G.1 Isolation and Clonal Assay of Adult Lung Epithelial Stem/Progenitor Cells
5. Chapter 3 Cancer Stem Cells& [5 X! Q. N$ D; |: U8 g
1. Unit 3.1 Colon Cancer Stem Cells
2. Unit 3.2 In Vivo Evaluation of Leukemic Stem Cells through the Xenotransplantation Model
3. Unit 3.3 Culture and Isolation of Brain Tumor Initiating Cells' Z* P/ V) Y. v. g9 T
6. Chapter 4 Manipulation of Potency8 o9 D8 x* }$ d# `9 f
1. Section A iPS Cells
1. Unit 4A.1 Human iPS Cell Derivation/Reprogramming
2. Unit 4A.2 Generation and Characterization of Human Induced Pluripotent Stem Cells9 ~1 }& q8 C) K% `  ^
2. Section B Nuclear Transfer9 J* |1 d, m# @
1. Unit 4B.1 Heterokaryon-Based Reprogramming for Pluripotency
7. Chapter 5 Genetic Manipulation of Stem Cells
1. Section A Lineage Tracers in Stem Cells- a( h5 `1 g8 D! b( K7 e
1. Unit 5A.1 Imaging Neural Stem Cell Fate in Mouse Model of Glioma# o9 w4 b1 G$ B/ a5 N" P& v
2. Unit 5A.2 Functional Analysis of Adult Stem Cells Using Cre-Mediated Lineage Tracing
3. Unit 5A.3 Magnetic Resonance Imaging of Human Embryonic Stem Cells
4. Unit 5A.4 Lineage Tracing in the Intestinal Epithelium: M/ b) v& L$ w  Z4 S
5. Unit 5A.5 Kaede-Centrin1 Labeling of Mother and Daughter Centrosomes in Mammalian" ?0 o6 e5 s: H: E2 m. Q+ K- X) H
Neocortical Neural Progenitors
2. Section B Homologous Recombination in Stem Cells. R! L0 j. x% c4 C
6. Unit 5B.1 Generation of Human Embryonic Stem Cell Reporter Knock-In Lines by7 i% @( ?9 S& K" U. A! z7 R
Homologous Recombination
8. Appendix 1 Useful Information( R! J: g2 a. ]/ ]
1. 1A Guidelines for the Conduct of Human Embryonic Stem Cell Research
2. 1B ISSCR Guidelines for the Clinical Translation of Stem Cells2 a; K% M6 K8 B- {* C% J
9. Appendix 2 Laboratory Equipment
Standard Laboratory Equipment
10. Appendix Suppliers% H1 j& o. w& u1 t' X& c( i
Selected Suppliers of Reagents and Equipment

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