PDF电子书：Current Protocols in Stem Cell Biology 2007年完整版

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6 K% u, {- d  ~8 S0 E/ F! HCurrent Protocols in Stem Cell Biology 2007年完整版 1451页
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Online ISBN:9780470151808; k4 [. ]! p. r) a' o2 ~- |
DOI: 10.1002/9780470151808# d- f  ?7 Q* [5 g4 \5 E* i
Table of Contents7 j! x  N$ n. v. V
1. Foreword
6 ?, Q5 J2 t* c; P9 C2. Preface
8 B* Y# |0 i' _8 N3. Chapter 1 Embryonic and Extraembryonic Stem Cells
. Q3 N) F& B1 x- A2 O9 n7 `" C& J+ H1. Section A Isolation of Embryonic Stem Cells9 {$ W! {2 c% W: u
1. Introduction' @3 ?8 g! ^& s- `" J! [& X) f8 i# h
2. Unit 1A.1 Derivation and Characterization of Nonhuman Primate Embryonic Stem Cells3 w+ k; U8 y1 j
3. Unit 1A.2 Derivation of hESC from Intact Blastocysts  V, O: k9 j9 h9 |' {! w
4. Unit 1A.3 Reprogramming Primordial Germ Cells (PGC) to Embryonic Germ (EG) Cells
; n5 U0 D8 T0 Q5. Unit 1A.4 Derivation and Propagation of hESC Under a Therapeutic Environment
% N* ^- y( J6 x) h# O" u% y6 r2. Section B Characterization of Embryonic Stem Cells
  b4 S1 Q0 }5 ?% I9 O& T1. Unit 1B.1 Proteomic Analysis of Pluripotent Stem Cells
! d5 _* O1 k; `, S& |! [% [  m2. Unit 1B.2 Gene Expression Analysis of RNA Purified from Embryonic Stem Cells and! e' ~1 r7 s- T5 Q7 x$ U# `/ w6 y
Embryoid Body–Derived Cells Using a High-Throughput Microarray Platform9 c  L/ j1 J% o$ j
3. Unit 1B.3 Phenotypic Analysis of Human Embryonic Stem Cells
% ^, Z, X/ d# D4 Z- E, m; O4. Unit 1B.4 Isolation of Human Embryonic Stem Cell–Derived Teratomas for the Assessment of. e2 g' e9 M+ O# h
Pluripotency) M. |$ E4 U5 ^9 y8 @. D
5. Unit 1B.5 Tandem Affinity Purification of Protein Complexes in Mouse Embryonic Stem Cells. Z/ [* |$ ]$ f9 `
Using In Vivo Biotinylation' H1 q7 S1 A' N1 J% l+ ^4 E1 k
6. Unit 1B.6 Characterization of X-Chromosome Inactivation Status in Human Pluripotent Stem' ~! R3 f* |2 T+ h, l
Cells; u! s2 G2 X4 D( G
7. Unit 1B.7 Preparation of Defined Human Embryonic Stem Cell Populations for Transcriptional
6 T: d- {" y4 J7 E/ L% n* RProfiling
1 `1 |! V5 Z" i3 \% D  P3. Section C Culture and Maintenance of Undifferentiated Embryonic Stem Cells
2 H$ C$ V% n1 K6 _" ?% f  r( @( z1. Introduction
; ~6 e& v1 e$ P2. Unit 1C.1 Expansion of Human Embryonic Stem Cells In Vitro
6 s! q- G  K- S; g# Q" Q3. Unit 1C.2 Defined, Feeder-Independent Medium for Human Embryonic Stem Cell Culture
) S0 z/ X* P5 v2 n4. Unit 1C.3 Isolation and Propagation of Mouse Embryonic Fibroblasts and Preparation of& U4 C, y- r4 c* n/ s
Mouse Embryonic Feeder Layer Cells
3 }; j/ y7 @  {/ o2 F0 b5. Unit 1C.4 Culture of Mouse Embryonic Stem Cells8 b9 R! ?. H& q2 S0 h
6. Unit 1C.5 Preparation of Autogenic Human Feeder Cells for Growth of Human Embryonic
0 ^4 Q+ i5 }% b1 e7 w/ W$ D* k2 uStem Cells
6 K9 A! [! |% W6 x( y7. Unit 1C.6 Isolation of Human Placental Fibroblasts
' G6 R% }+ o& @" {8. Unit 1C.7 Derivation of Human Skin Fibroblast Lines for Feeder Cells of Human Embryonic
0 ]2 P  c& p& g  a5 eStem Cells& Y( s4 g6 E0 Y% S& ?
9. Unit 1C.8 Cryopreservation of Dissociated Human Embryonic Stem Cells in the Presence of+ e) [, P7 H4 c4 E4 J+ C2 `1 p
ROCK Inhibitor
0 R$ ]( w9 o+ Z10. Unit 1C.9 Authentication and Banking of Human Pluripotent Stem Cells3 F; J, ]( q& l
11. Unit 1C.10 Clump Passaging and Expansion of Human Embryonic and Induced Pluripotent
' q2 [' X4 U1 [. bStem Cells on Mouse Embryonic Fibroblast Feeder Cells
9 a" k8 m2 l( _12. Unit 1C.11 Expansion of Human Embryonic Stem Cells on Cellulose Microcarriers
, W8 ^- y/ c" R% Q! v4. Section D Germ Layer Induction/Differentiation of Embryonic Stem Cells6 }$ {+ v  y# W  R8 `; v9 B
1. Unit 1D.1 Germ Layer Induction in ESC—Following the Vertebrate Roadmap! D( @1 }& Z# L0 s% {1 E
2. Unit 1D.2 Formation and Hematopoietic Differentiation of Human Embryoid Bodies by
# b& A. u- W( b1 J  t4 m( {/ vSuspension and Hanging Drop Cultures5 e0 D& I7 K: E3 O" a6 Y' q: j
3. Unit 1D.3 Directed Differentiation of Human Embryonic Stem Cells as Spin Embryoid Bodies
  a  O) L' D: ?, i/ K9 ?$ tand a Description of the Hematopoietic Blast Colony Forming Assay
' c( p# q. ~; {; E4. Unit 1D.4 Differentiation of Human Embryonic Stem Cells in Adherent and in Chemically: p, M1 e) K7 d5 @5 k$ _' B
Defined Culture Conditions6 g. A$ l- |1 S% h
5. Unit 1D.5 Isolation and Differentiation of Xenopus Animal Cap Cells3 A8 h& q$ [2 j' L" @1 ^# ?5 Q4 n
5. Section E Extraembryonic Lineages# e+ Z/ w& y2 m4 h. K7 ?  A0 g
1. Introduction
% c+ C6 C. {3 j4 O$ {2. Unit 1E.1 Isolation of Human Placenta-Derived Multipotent Cells and In Vitro Differentiation9 u" l9 s0 a6 s
into Hepatocyte-Like Cells
( X# `2 \  g7 r- d! a3. Unit 1E.2 Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta
; N; K, ~6 {; h4. Unit 1E.3 Isolation of Amniotic Epithelial Stem Cells
+ X; x0 K. G0 z  H' _; _5. Unit 1E.4 Isolation and Manipulation of Mouse Trophoblast Stem Cells
! ]7 E1 l3 H+ M3 C, @% [+ }7 q6. Unit 1E.5 Isolation of Amniotic Mesenchymal Stem Cells1 K+ Q+ ~, G/ i2 G+ x
7. Unit 1E.6 Amnion Epithelial Cell Isolation and Characterization for Clinical Use" Q; I2 m( Z; j9 _2 Q; l) _1 W
6. Section F Mesodermal Lineages
, M. ~" d8 v+ P1. Unit 1F.1 Differentiation of Embryonic Stem Cells into Cartilage Cells8 S+ o( H8 F: U, }# c$ m% @/ ?
2. Unit 1F.2 Differentiation of Human Embryonic Stem Cells to Cardiomyocytes by Coculture7 c  p2 w2 _2 @, C
with Endoderm in Serum-Free Medium) h4 G, L3 [6 s2 \6 W7 C+ s
3. Unit 1F.3 Isolation of Hematopoietic Stem Cells from Mouse Embryonic Stem Cells; _1 v: f- g, ~* d; t: w, z
4. Unit 1F.4 Differentiation of Mouse Embryonic Stem Cells into Blood% ]3 Q% Z/ [+ @
5. Unit 1F.5 Endothelial Differentiation of Embryonic Stem Cells
; R) f! A, ?$ P. J+ C3 q% _8 l6. Unit 1F.6 Hematopoietic Differentiation of Human Embryonic Stem Cells by Cocultivation# H/ H# h5 |) M7 `& N+ {, m
with Stromal Layers1 c$ d8 p. l" o4 y4 y8 ?: z
7. Unit 1F.7 TLX1 (HOX11) Immortalization of Embryonic Stem Cell–Derived and Primary
( Y. S& d: Z. m1 b3 k8 rMurine Hematopoietic Progenitors
9 L0 ]( Z1 n/ ^& U& Q5 w3 C8. Unit 1F.8 Differentiation of Multipotent Mesenchymal Precursors and Skeletal Myoblasts from
4 g. E) W% W% a) QHuman Embryonic Stem Cells
8 c0 E! f, D, u' A9. Unit 1F.9 Derivation of Vasculature from Embryonic Stem Cells, A' j6 M2 m; N' ~8 f
10. Unit 1F.10 Isolation and Functional Characterization of Pluripotent Stem Cell–Derived Cardiac
+ Z# S3 P2 k) E4 F3 IProgenitor Cells
" s8 M% B* v4 }$ i11. Unit 1F.11 Differentiation of Mouse Embryonic Stem Cells into Cardiomyocytes via the
$ x: d* v& g! z5 \+ H  z! K/ QHanging-Drop and Mass Culture Methods1 m8 }0 I# X- i2 ?
7. Section G Endodermal Lineages
& F  |' F- t! @5 O3 K5 W- |. @, T1. Unit 1G.1 The Differentiation of Distal Lung Epithelium from Embryonic Stem Cells8 l6 S6 x( h# d
2. Unit 1G.2 Pancreas Differentiation of Mouse ES Cells8 B# Z7 @+ |/ `7 U4 o6 l  D$ }$ \
3. Unit 1G.3 Differentiation of Embryonic Stem Cells into Anterior Definitive Endoderm9 n. i- `9 Z% g' i+ T# V/ t2 ]1 w
8. Section H Ectodermal Lineages$ _+ N2 X0 V' x3 g7 E. V
1. Unit 1H.1 Differentiation of Mouse Embryonic Stem Cells to Spinal Motor Neurons# w7 h% b. T9 i" c! X3 @0 a* s" w9 {. J
2. Unit 1H.2 Time-Lapse Imaging of Embryonic Neural Stem Cell Division in Drosophila by$ ?3 Z9 B% h1 r/ O
Two-Photon Microscopy' f* T* H! u  r) a5 F
4. Chapter 2 Somatic Stem Cells
3 [6 P. u, T: X1. Section A Hematopoietic Stem Cells' [- i- R# A+ P/ f2 F
1. Introduction6 Q2 w* P  {  B' t$ U! s6 _6 J* \- S
2. Unit 2A.1 Isolation of Mononuclear Cells from Human Cord Blood by Ficoll-Paque Density
' d- \( i0 ^* H- zGradient
8 A0 l# j8 ?1 J2 d8 S0 z2 s3. Unit 2A.2 Isolation of Hematopoietic Stem Cells from Human Cord Blood
' b+ E$ b/ Y1 R: Y, B$ R. c4. Unit 2A.3 Isolation of Mesenchymal Stem Cells from Human Cord Blood# R8 \( a: c) g1 L  a4 }0 O
5. Unit 2A.4 Isolation and Assessment of Long-Term Reconstituting Hematopoietic Stem Cells' P4 P& M9 N* m
from Adult Mouse Bone Marrow3 O$ v+ R8 R; f2 }$ g  H$ f
6. Unit 2A.5 Analysis of the Hematopoietic Stem Cell Niche
* W+ G- t) ?9 q$ H8 D- V# X7. Unit 2A.6 Analysis and Manipulation of Hematopoietic Progenitor and Stem Cells from Murine! M9 h9 j* J2 c# A1 J" p( V2 n
Embryonic Tissues9 T+ t+ z5 B! v! x& d
8. Unit 2A.7 High Level In Vitro Expansion of Murine Hematopoietic Stem Cells$ s) \2 k- ^# ]' G9 @* \
9. Unit 2A.8 Isolation and Visualization of Mouse Placental Hematopoietic Stem Cells2 N$ v; O- T' M
10. Unit 2A.9 Preparation of Hematopoietic Stem and Progenitor Cells from the Human Placenta  {. r6 I: e+ L& e
2. Section B Non-Hematopoietic Bone Marrow-Derived Stem Cells
) P+ t: r$ M+ V3 Z0 Q0 L( [1. Unit 2B.1 Isolation and Characterization of Mesoangioblasts from Mouse, Dog, and Human+ T1 x# \+ Q0 j7 |/ h& c# j5 M
Tissues5 K" J' G! h7 R4 {4 v1 N
2. Unit 2B.2 Purification and Culture of Human Blood Vessel–Associated Progenitor Cells
8 ^# p+ g! v, n$ H# ^5 L+ u1 Q0 u3. Unit 2B.3 Isolation, Culture, and Differentiation Potential of Mouse Marrow Stromal Cells7 q: r8 I- ~' F5 V3 [# A* D2 @
3. Section C Cardiovascular Stem Cells
( j. _# g  m, @. ?, F1. Unit 2C.1 Isolation and Characterization of Endothelial Progenitor Cells from Human Blood& o& t- X4 q# _/ \) E0 C0 r8 G
2. Unit 2C.2 Derivation of Epicardium-Derived Progenitor Cells (EPDCs) from Adult Epicardium# q, s2 b8 s' k
3. Unit 2C.3 Isolation and Expansion of Cardiosphere-Derived Stem Cells. o- I3 ]6 n& {) v: D0 K
4. Section D Neural Stem Cells9 T3 H) y' u* W2 E2 }
1. Unit 2D.1 Generation of Cultured Oligodendrocyte Progenitor Cells from Rat Neonatal Brains
5 }, \! h  m+ M  G: s2. Unit 2D.2 Isolating, Expanding, and Infecting Human and Rodent Fetal Neural Progenitor Cells
/ G! x5 @# _1 p, P( B5 L6 F- f3. Unit 2D.3 Long-Term Multilayer Adherent Network (MAN) Expansion, Maintenance, and6 K' \" t$ {. e
Characterization, Chemical and Genetic Manipulation, and Transplantation of Human Fetal
; d! y" _% k7 G+ m+ i% xForebrain Neural Stem Cells
7 V7 u! w; B# L1 i4. Unit 2D.4 Culture System for Rodent and Human Oligodendrocyte Specification, Lineage& f9 s* B+ K0 r; b# U! G4 D5 C
Progression, and Maturation
' |( D3 \  b, H" N9 K9 ~# @5. Unit 2D.5 Isolation and Culture of Ventral Mesencephalic Precursor Cells and Dopaminergic
/ p/ ]8 s3 h- ZNeurons from Rodent Brains- p0 f# E( V5 Q! n' q7 C$ ~4 b% v+ N. S& w0 m
6. Unit 2D.6 Isolation of Neural Stem Cells from Neural Tissues Using the Neurosphere- h+ R6 c* J' E) s) {, G% q; Z
Technique
, Q1 y7 D* j) B5 ~5. Section E Germline Stem Cells
( c0 V& D& b3 b8 M: e1. Unit 2E.1 Culturing Ovarian Somatic and Germline Stem Cells of Drosophila5 e* w7 C4 ]0 `+ d$ V# O
2. Unit 2E.2 Time-Lapse Live Imaging of Stem Cells in Drosophila Testis' n, _' w- O: |- g* @$ y. [, P- o* Y
6. Section F Gut Stem Cells( A( f, T' [' r: y
1. Unit 2F.1 In Situ Hybridization to Identify Gut Stem Cells
" Z7 v# `  i8 m7. Section G Lung Stem Cells* W! ^! E  Y' K8 n' B0 b5 x
1. Unit 2G.1 Isolation and Clonal Assay of Adult Lung Epithelial Stem/Progenitor Cells
8 ^) A; Q6 U% V5 w5. Chapter 3 Cancer Stem Cells$ T' c: {0 M) v; k. r' X4 N2 u
1. Unit 3.1 Colon Cancer Stem Cells
3 K5 ?0 }6 W9 O1 {2 I& G2. Unit 3.2 In Vivo Evaluation of Leukemic Stem Cells through the Xenotransplantation Model5 f. g9 w9 H) r2 M' I# O" r
3. Unit 3.3 Culture and Isolation of Brain Tumor Initiating Cells
) I6 N0 r7 W/ |2 r5 ]6. Chapter 4 Manipulation of Potency/ u# T) M+ T9 U+ O' |
1. Section A iPS Cells  K! \+ I7 Z% t; D  W; Z. W6 N
1. Unit 4A.1 Human iPS Cell Derivation/Reprogramming# q  e' P0 R# d: z, |+ a% v
2. Unit 4A.2 Generation and Characterization of Human Induced Pluripotent Stem Cells/ H' n: A- q( ^3 p/ P) y# f/ d
2. Section B Nuclear Transfer
4 L; r! w$ z: `9 S; w1. Unit 4B.1 Heterokaryon-Based Reprogramming for Pluripotency& R3 W' G, l: U. _; Z2 @
7. Chapter 5 Genetic Manipulation of Stem Cells
& c/ B0 h  Z% t; G3 D+ J# L5 r1. Section A Lineage Tracers in Stem Cells( S' Z* ?* q9 C
1. Unit 5A.1 Imaging Neural Stem Cell Fate in Mouse Model of Glioma/ n" z6 [+ U1 w' o
2. Unit 5A.2 Functional Analysis of Adult Stem Cells Using Cre-Mediated Lineage Tracing
: V# H0 `3 d# C( ~1 J  X- y3. Unit 5A.3 Magnetic Resonance Imaging of Human Embryonic Stem Cells
4 u. e  c4 w7 y( G# [9 I8 f+ ~+ M4. Unit 5A.4 Lineage Tracing in the Intestinal Epithelium% y8 f3 e" h, Z3 M& n
5. Unit 5A.5 Kaede-Centrin1 Labeling of Mother and Daughter Centrosomes in Mammalian+ P9 L5 R9 ^: w& ^+ h' A
Neocortical Neural Progenitors
8 h  i2 U: a* u: u" U$ J# n2. Section B Homologous Recombination in Stem Cells, |7 d, _. \5 ]# b8 S& @$ R
6. Unit 5B.1 Generation of Human Embryonic Stem Cell Reporter Knock-In Lines by
- E) [0 m8 Q7 A( l0 A$ ^Homologous Recombination# [! L4 O; Q) Z2 q" M3 I
8. Appendix 1 Useful Information
- q2 {. i- C( ^' P$ [( B, M1. 1A Guidelines for the Conduct of Human Embryonic Stem Cell Research) E8 s  f$ q( F
2. 1B ISSCR Guidelines for the Clinical Translation of Stem Cells! R1 S- E: Y# Z8 Y3 O  {$ N: h- Z
9. Appendix 2 Laboratory Equipment
% R$ z8 B( J0 h) I. Z% BStandard Laboratory Equipment4 ]8 U! O8 D) c* v4 |
10. Appendix Suppliers+ Y9 a. y. z4 x: a( f. _1 o
Selected Suppliers of Reagents and Equipment
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