PDF电子书：Current Protocols in Stem Cell Biology 2007年完整版

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Current Protocols in Stem Cell Biology 2007年完整版 1451页9 z. s8 q& N2 b
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Online ISBN:9780470151808
  e2 Z( V0 ?& }, k$ uDOI: 10.1002/9780470151808
  r8 p: `: H$ p: ?( a* k" DTable of Contents
4 R  ]; D; K4 q1 W1. Foreword
) R$ D" ]$ N1 c. g2 Y1 G; J2. Preface8 o0 R% F- h* z2 a. `
3. Chapter 1 Embryonic and Extraembryonic Stem Cells& c) g% _3 r" v2 j
1. Section A Isolation of Embryonic Stem Cells9 T, Z, b, ?7 c
1. Introduction
7 {6 Y, \, Q: m2. Unit 1A.1 Derivation and Characterization of Nonhuman Primate Embryonic Stem Cells3 f# M8 }7 [6 v+ X5 l
3. Unit 1A.2 Derivation of hESC from Intact Blastocysts
) _$ \; O; s! M' M6 E- o) P; \( Z4. Unit 1A.3 Reprogramming Primordial Germ Cells (PGC) to Embryonic Germ (EG) Cells# {5 T" E. s9 `  ?" _
5. Unit 1A.4 Derivation and Propagation of hESC Under a Therapeutic Environment
4 {4 L1 s0 j. v+ {0 \8 z+ O2. Section B Characterization of Embryonic Stem Cells
) Q9 m, _5 C5 X# G1. Unit 1B.1 Proteomic Analysis of Pluripotent Stem Cells; J& c' H/ f& Z* B- f5 q
2. Unit 1B.2 Gene Expression Analysis of RNA Purified from Embryonic Stem Cells and9 E3 m! p9 a# L* {# F
Embryoid Body–Derived Cells Using a High-Throughput Microarray Platform
* p8 O" H/ @* Y7 F3. Unit 1B.3 Phenotypic Analysis of Human Embryonic Stem Cells
8 t) T* g+ Q* G# u8 ~6 M4. Unit 1B.4 Isolation of Human Embryonic Stem Cell–Derived Teratomas for the Assessment of  u6 P' @0 J# C  u7 H
Pluripotency
7 S* f; a0 V8 x5. Unit 1B.5 Tandem Affinity Purification of Protein Complexes in Mouse Embryonic Stem Cells) ^& x4 O8 Z5 b! C' m
Using In Vivo Biotinylation
( k" {; Y  f. {4 v9 _- |( j6. Unit 1B.6 Characterization of X-Chromosome Inactivation Status in Human Pluripotent Stem1 ~7 E, y0 w$ P1 O
Cells& j9 _4 ?; `% V( v
7. Unit 1B.7 Preparation of Defined Human Embryonic Stem Cell Populations for Transcriptional
; b9 t' l1 Q$ d8 {9 iProfiling
7 E+ `! j; t: {0 }, ^% m& f9 `* U3. Section C Culture and Maintenance of Undifferentiated Embryonic Stem Cells
% C! H. I5 `' |' }8 Q6 ?/ |5 ?0 B1. Introduction6 o6 G' S/ j* ?/ G1 P% n* `4 W. ^
2. Unit 1C.1 Expansion of Human Embryonic Stem Cells In Vitro
' z" s$ Q) K9 q! y. V  z+ y6 n# h% K5 m3. Unit 1C.2 Defined, Feeder-Independent Medium for Human Embryonic Stem Cell Culture& L" s: k6 s, Z  @' e" _5 Z
4. Unit 1C.3 Isolation and Propagation of Mouse Embryonic Fibroblasts and Preparation of9 ]4 ?/ k$ {2 L- [- B
Mouse Embryonic Feeder Layer Cells  o/ J" p4 m% l4 _
5. Unit 1C.4 Culture of Mouse Embryonic Stem Cells2 A6 a  Z6 ?# m* E
6. Unit 1C.5 Preparation of Autogenic Human Feeder Cells for Growth of Human Embryonic. ]8 H7 M' Z& Y$ w4 U* M+ O' p
Stem Cells
( d7 F: ~. A; v; x7. Unit 1C.6 Isolation of Human Placental Fibroblasts
4 c! E3 H5 a  n8. Unit 1C.7 Derivation of Human Skin Fibroblast Lines for Feeder Cells of Human Embryonic  n$ B8 o1 g2 E1 r) Q4 s! f8 k
Stem Cells* L6 ~& F0 W6 X6 R- v+ N
9. Unit 1C.8 Cryopreservation of Dissociated Human Embryonic Stem Cells in the Presence of
4 L# M; o* L: h  KROCK Inhibitor; M& Q0 `! t+ q% V
10. Unit 1C.9 Authentication and Banking of Human Pluripotent Stem Cells* T9 ?3 ^/ ?$ t0 L
11. Unit 1C.10 Clump Passaging and Expansion of Human Embryonic and Induced Pluripotent0 l7 o3 ^6 I" T( H9 h( X  f$ W9 W
Stem Cells on Mouse Embryonic Fibroblast Feeder Cells  i, R, M* f8 ~! o
12. Unit 1C.11 Expansion of Human Embryonic Stem Cells on Cellulose Microcarriers3 g5 ~3 a- ~. O
4. Section D Germ Layer Induction/Differentiation of Embryonic Stem Cells
+ Y, B4 D$ Z+ \1. Unit 1D.1 Germ Layer Induction in ESC—Following the Vertebrate Roadmap
# p* ~2 n/ _6 p" N" c* y8 ]8 d; ^2. Unit 1D.2 Formation and Hematopoietic Differentiation of Human Embryoid Bodies by1 _! k9 \& _; y, Q8 W6 l
Suspension and Hanging Drop Cultures
! n4 m$ P' Y1 j& d# e# d$ K3. Unit 1D.3 Directed Differentiation of Human Embryonic Stem Cells as Spin Embryoid Bodies
* C, \% z. V4 Y8 ?and a Description of the Hematopoietic Blast Colony Forming Assay% C5 {2 Z% _, y
4. Unit 1D.4 Differentiation of Human Embryonic Stem Cells in Adherent and in Chemically2 _' ^* p, z; Q1 \" o3 K1 Z
Defined Culture Conditions0 H  r% G% [. e, B; U- p
5. Unit 1D.5 Isolation and Differentiation of Xenopus Animal Cap Cells
) p  m$ {& s9 k7 o& P7 q5. Section E Extraembryonic Lineages
0 M: Y5 Z, V$ I6 e4 }1. Introduction: }/ X/ e: V% c4 j- z, K  A* A
2. Unit 1E.1 Isolation of Human Placenta-Derived Multipotent Cells and In Vitro Differentiation
0 g: E/ B2 {* _" ]/ Kinto Hepatocyte-Like Cells
( M% y9 o3 @; o5 g: l. l( T' V3. Unit 1E.2 Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta: t5 [9 b: ^' r. R) t# J
4. Unit 1E.3 Isolation of Amniotic Epithelial Stem Cells
+ }/ N5 K& q8 h5. Unit 1E.4 Isolation and Manipulation of Mouse Trophoblast Stem Cells
+ y# o9 W8 F0 O& Y$ j* N0 Y6. Unit 1E.5 Isolation of Amniotic Mesenchymal Stem Cells
0 k9 n% C5 C) T2 ?: d7. Unit 1E.6 Amnion Epithelial Cell Isolation and Characterization for Clinical Use" V' Z" h/ r( h* z8 Z* U  q
6. Section F Mesodermal Lineages, W; _1 t" B! O! |# w
1. Unit 1F.1 Differentiation of Embryonic Stem Cells into Cartilage Cells8 H- p! r: u, _; z0 O
2. Unit 1F.2 Differentiation of Human Embryonic Stem Cells to Cardiomyocytes by Coculture
7 c5 r# B2 T- [: Bwith Endoderm in Serum-Free Medium
% N+ a8 T- a* E  U3. Unit 1F.3 Isolation of Hematopoietic Stem Cells from Mouse Embryonic Stem Cells
2 x2 _( `: P" K7 t: s" w. I4 `  q4. Unit 1F.4 Differentiation of Mouse Embryonic Stem Cells into Blood
1 F. G' T1 j8 {- m4 B5. Unit 1F.5 Endothelial Differentiation of Embryonic Stem Cells
4 P( x! g. m. u" K8 F! u* q6. Unit 1F.6 Hematopoietic Differentiation of Human Embryonic Stem Cells by Cocultivation) G% o- T& H0 Q& U5 L
with Stromal Layers# c1 K0 q$ q" |  h! Q) y( p8 X
7. Unit 1F.7 TLX1 (HOX11) Immortalization of Embryonic Stem Cell–Derived and Primary
) Q! g9 @% X9 B2 I; E7 h# R9 VMurine Hematopoietic Progenitors
8 H1 o; ]7 P0 T  J1 q8. Unit 1F.8 Differentiation of Multipotent Mesenchymal Precursors and Skeletal Myoblasts from
. h5 V$ a0 b* G; w6 _0 hHuman Embryonic Stem Cells. [8 s; r" G, \
9. Unit 1F.9 Derivation of Vasculature from Embryonic Stem Cells
- A' |+ h3 Q; }* d) V9 `10. Unit 1F.10 Isolation and Functional Characterization of Pluripotent Stem Cell–Derived Cardiac
7 y$ c0 p% y% V  |Progenitor Cells
- e5 n! ?% j( M( v) _+ @11. Unit 1F.11 Differentiation of Mouse Embryonic Stem Cells into Cardiomyocytes via the
, g8 r0 p( a. h2 C( uHanging-Drop and Mass Culture Methods% C& K$ B. C6 W; q# r
7. Section G Endodermal Lineages
# K1 u0 S4 K8 ]1. Unit 1G.1 The Differentiation of Distal Lung Epithelium from Embryonic Stem Cells* G6 A$ h& j; Y2 _6 Q9 N/ H, a6 i! e
2. Unit 1G.2 Pancreas Differentiation of Mouse ES Cells+ \9 k  N, ^" i- v6 y/ S( U9 s
3. Unit 1G.3 Differentiation of Embryonic Stem Cells into Anterior Definitive Endoderm
2 A! E( J; T+ ~* H: C* m8. Section H Ectodermal Lineages
  B& D4 E9 S5 A9 @$ F( z1. Unit 1H.1 Differentiation of Mouse Embryonic Stem Cells to Spinal Motor Neurons
7 V- E* b9 C$ s" k, A! p7 }1 |2. Unit 1H.2 Time-Lapse Imaging of Embryonic Neural Stem Cell Division in Drosophila by  a7 g# u' i% g) y7 |: t" ?
Two-Photon Microscopy
1 e" Q6 O- X/ H: `4. Chapter 2 Somatic Stem Cells4 G( V2 J% N+ @
1. Section A Hematopoietic Stem Cells
8 f; U9 ]- x! q7 {1. Introduction& X) G( P3 Y% s8 F' U1 y' L
2. Unit 2A.1 Isolation of Mononuclear Cells from Human Cord Blood by Ficoll-Paque Density% ]: \0 U# I+ o" b) v
Gradient
. m& o( A: n1 w, ^- n: _, |3. Unit 2A.2 Isolation of Hematopoietic Stem Cells from Human Cord Blood
* O% Q; \4 J: J+ w+ z3 _+ [4. Unit 2A.3 Isolation of Mesenchymal Stem Cells from Human Cord Blood: W  _, a7 }# O6 T- U
5. Unit 2A.4 Isolation and Assessment of Long-Term Reconstituting Hematopoietic Stem Cells2 A9 h/ [' k+ E2 k8 v
from Adult Mouse Bone Marrow% H/ y7 P! W6 g9 B8 }
6. Unit 2A.5 Analysis of the Hematopoietic Stem Cell Niche7 ^4 r5 ?) W+ l! R
7. Unit 2A.6 Analysis and Manipulation of Hematopoietic Progenitor and Stem Cells from Murine. U8 `! m" a* ?! L- v
Embryonic Tissues" ^; ~: M. W: f2 H) s8 K: W
8. Unit 2A.7 High Level In Vitro Expansion of Murine Hematopoietic Stem Cells9 e! }* @" X( q8 H
9. Unit 2A.8 Isolation and Visualization of Mouse Placental Hematopoietic Stem Cells& _7 Z: S3 j1 ?$ t  y+ u6 Q2 p! b
10. Unit 2A.9 Preparation of Hematopoietic Stem and Progenitor Cells from the Human Placenta
3 r- }; S3 ?+ t! C& Z& x. M: ?2. Section B Non-Hematopoietic Bone Marrow-Derived Stem Cells
% [' V, H4 g! H" M7 ^1 g3 t1. Unit 2B.1 Isolation and Characterization of Mesoangioblasts from Mouse, Dog, and Human
$ J0 P# ]' b3 @" J& F5 m3 q6 hTissues. r7 ?1 Z$ U' f
2. Unit 2B.2 Purification and Culture of Human Blood Vessel–Associated Progenitor Cells
) H. \' `7 p( ~- o; ]' N( f3. Unit 2B.3 Isolation, Culture, and Differentiation Potential of Mouse Marrow Stromal Cells( ^) o6 u3 z  }8 I; F* p
3. Section C Cardiovascular Stem Cells: D0 o' L* ~1 c7 v& _& z
1. Unit 2C.1 Isolation and Characterization of Endothelial Progenitor Cells from Human Blood
% G( c9 }) L" r1 f2. Unit 2C.2 Derivation of Epicardium-Derived Progenitor Cells (EPDCs) from Adult Epicardium
" h/ ^6 b& @9 ?- h) r) N3. Unit 2C.3 Isolation and Expansion of Cardiosphere-Derived Stem Cells
+ j: f% V0 S* `  r4. Section D Neural Stem Cells
4 {( l$ f6 n3 h; j1. Unit 2D.1 Generation of Cultured Oligodendrocyte Progenitor Cells from Rat Neonatal Brains/ g$ I) |: h0 x; o
2. Unit 2D.2 Isolating, Expanding, and Infecting Human and Rodent Fetal Neural Progenitor Cells" c5 `8 w6 |  U6 {* m0 A& V
3. Unit 2D.3 Long-Term Multilayer Adherent Network (MAN) Expansion, Maintenance, and3 ]; W* k6 ^0 M$ G( Q# R2 ~  f
Characterization, Chemical and Genetic Manipulation, and Transplantation of Human Fetal
, c& Q$ t) ?/ T0 i* vForebrain Neural Stem Cells3 Y- e- f3 v* i; g
4. Unit 2D.4 Culture System for Rodent and Human Oligodendrocyte Specification, Lineage* O0 `* z' f% s- f
Progression, and Maturation
/ J1 I! @  D1 p$ ~  b" I5. Unit 2D.5 Isolation and Culture of Ventral Mesencephalic Precursor Cells and Dopaminergic$ q0 B) V8 p2 {, O
Neurons from Rodent Brains+ U+ U2 ]( l5 L6 z! Q* I/ z
6. Unit 2D.6 Isolation of Neural Stem Cells from Neural Tissues Using the Neurosphere9 t" i8 w3 b2 w
Technique9 P* }' w; N: t& ?9 X
5. Section E Germline Stem Cells3 b5 \5 i/ c: p7 f- n% v! V* X
1. Unit 2E.1 Culturing Ovarian Somatic and Germline Stem Cells of Drosophila
  P6 s; G0 E$ Q9 Z& c, [2. Unit 2E.2 Time-Lapse Live Imaging of Stem Cells in Drosophila Testis' O& B" g8 _$ c8 v, \/ e! T
6. Section F Gut Stem Cells0 W- p0 J% s. ]( _
1. Unit 2F.1 In Situ Hybridization to Identify Gut Stem Cells
0 q- L' Y" J( U+ u8 Y. N- n" x; f7. Section G Lung Stem Cells
8 `5 s, d9 G: C' c1. Unit 2G.1 Isolation and Clonal Assay of Adult Lung Epithelial Stem/Progenitor Cells
' \2 P% L* T" N3 R' h' K5. Chapter 3 Cancer Stem Cells/ g5 V( f* g+ y- @
1. Unit 3.1 Colon Cancer Stem Cells- T- f3 W( M/ M$ E
2. Unit 3.2 In Vivo Evaluation of Leukemic Stem Cells through the Xenotransplantation Model# H* V! ^/ D6 F' C3 N4 B
3. Unit 3.3 Culture and Isolation of Brain Tumor Initiating Cells
& o; T6 c6 [- `* K6. Chapter 4 Manipulation of Potency( W/ f( i/ l0 @! I0 B0 T) W# }6 h* h
1. Section A iPS Cells  j; m) `) ^! J( ?3 e: k0 Q
1. Unit 4A.1 Human iPS Cell Derivation/Reprogramming' r' t2 J7 q) H
2. Unit 4A.2 Generation and Characterization of Human Induced Pluripotent Stem Cells
! y# }3 a; p( u6 `: g0 _2. Section B Nuclear Transfer' J6 V% `2 i( T0 G: ^
1. Unit 4B.1 Heterokaryon-Based Reprogramming for Pluripotency8 N' i8 V$ |8 m2 W. v
7. Chapter 5 Genetic Manipulation of Stem Cells& b) G8 D0 z4 }/ Y. P
1. Section A Lineage Tracers in Stem Cells
0 r* O  Y, u) i4 K0 T1. Unit 5A.1 Imaging Neural Stem Cell Fate in Mouse Model of Glioma6 {, o+ F: u! r9 \
2. Unit 5A.2 Functional Analysis of Adult Stem Cells Using Cre-Mediated Lineage Tracing4 `% K* k1 o3 J
3. Unit 5A.3 Magnetic Resonance Imaging of Human Embryonic Stem Cells# {% a3 ~: L7 e2 q  R
4. Unit 5A.4 Lineage Tracing in the Intestinal Epithelium
8 n% L. W( [5 K1 Z* o: v5. Unit 5A.5 Kaede-Centrin1 Labeling of Mother and Daughter Centrosomes in Mammalian
/ R$ g7 o; D' w- O# I+ j/ A* MNeocortical Neural Progenitors+ F; y( \5 Z# b# r
2. Section B Homologous Recombination in Stem Cells
' n3 g+ R& T& s1 x; ]- T1 ^6 q2 e6. Unit 5B.1 Generation of Human Embryonic Stem Cell Reporter Knock-In Lines by
$ L$ Y2 _) `, C$ MHomologous Recombination7 N3 B% P; U) Y& \3 w* K8 H% ?
8. Appendix 1 Useful Information
3 ]2 a6 H/ [, J  E( L: i1. 1A Guidelines for the Conduct of Human Embryonic Stem Cell Research$ E' D4 p+ U7 [3 D, E5 h
2. 1B ISSCR Guidelines for the Clinical Translation of Stem Cells$ i' b( ]9 D; x0 ^8 }$ X* O; W
9. Appendix 2 Laboratory Equipment
. R+ `' n1 U  k8 Q4 t3 ZStandard Laboratory Equipment; @; d5 b/ h! C0 i2 |, N  T
10. Appendix Suppliers# w- Z- n: _9 w2 e3 ~4 n; Z
Selected Suppliers of Reagents and Equipment
6 ]$ y. L3 V, R9 \$ i" F5 ?. t. O8 J% @  a' [& \

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