求助：肝细胞的流式分离

wanglibin

最近要用流式的方法来分离小鼠的成体肝细胞，请问能用哪些抗体来进行？请大家帮帮忙

naturalkillerce

回复 wanglibin 的帖子# b" c/ F5 f3 n7 \

) g3 Z2 N" L7 Z9 h. @' \Isolated mouse hepatocyte purity check was introduced to monitor the expression of CD95 on hepatocytes using flow cytometry methods.
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, L+ N* j. x: R- m( X0 L( ~因此，CD95抗体应该是需要的。

naturalkillerce

回复 wanglibin 的帖子  P2 I4 o( Y4 D" w# G* A4 T4 m5 n
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给你提供一篇文献《Improved isolation of murine hepatocytes for in vitro malaria liver stage studies》供参考：8 s. i" K0 L) T% g
摘要为：
/ x! A0 `5 ~4 U6 j; eThis protocol is a detailed description of a technique to isolate and culture mouse
- P# q( R4 V0 m3 Mhepatocytes and represents an improvement over previous descriptions of hepatocyte isolation for
) e6 Y; u, Y, m0 _malaria studies, regarding three technical aspects: (1) dissociation reagents choice; (2) cell2 r) l  }8 D9 X* ^
separation gradient and (3) cell purity control. Cell dissociation was optimized for a specific
& A& }5 j9 p8 N, fcollagenase digestion media. The cell dissociation step was improved by using a three-layer
$ Q1 z3 C* z8 C8 ~7 Zdiscontinuous gradient. A cell purity check was introduced to monitor the expression of CD95 on/ T) e; x* h% Z! u
hepatocytes using flow cytometry methods.
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1475-2875-6-169.pdf (632.31 KB)

2011-11-15 15:04 上传

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wanglibin

回复 naturalkillerce 的帖子
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谢谢你了~