求助：肝细胞的流式分离

wanglibin

最近要用流式的方法来分离小鼠的成体肝细胞，请问能用哪些抗体来进行？请大家帮帮忙

naturalkillerce

回复 wanglibin 的帖子* V# Z  P) b. x/ {
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Isolated mouse hepatocyte purity check was introduced to monitor the expression of CD95 on hepatocytes using flow cytometry methods.
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6 e" }: M) r$ P8 k3 G; Y因此，CD95抗体应该是需要的。

naturalkillerce

回复 wanglibin 的帖子0 C5 ~: Y1 Y5 Q) T# r; H

# g# v/ r  \( z给你提供一篇文献《Improved isolation of murine hepatocytes for in vitro malaria liver stage studies》供参考：. }5 b# F  R4 k; R1 l6 W
摘要为：! |! L% |- x1 }6 s- e
This protocol is a detailed description of a technique to isolate and culture mouse1 d: w) U/ o% k* d- y  {5 ~+ s' s' u
hepatocytes and represents an improvement over previous descriptions of hepatocyte isolation for3 ?' s4 P, _8 E7 V5 b" \
malaria studies, regarding three technical aspects: (1) dissociation reagents choice; (2) cell' ^0 n2 I: p+ p  c1 J
separation gradient and (3) cell purity control. Cell dissociation was optimized for a specific% c( R9 E" _2 S# A2 U+ q0 q
collagenase digestion media. The cell dissociation step was improved by using a three-layer* N+ c1 s- u9 x
discontinuous gradient. A cell purity check was introduced to monitor the expression of CD95 on
; X% K6 r1 ^2 z- `  K3 n  ]) K4 lhepatocytes using flow cytometry methods.- O8 W. z. v7 ~. C: w

5 f. b2 Y: I/ K, i- Y8 X& w 1475-2875-6-169.pdf (632.31 KB)

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wanglibin

回复 naturalkillerce 的帖子
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谢谢你了~